Topical Photodynamic Therapy Mediated with Hematoporphyrin-HCl using Glycerin as Penetrating Enhancer: In vitro and in vivo studies

Document Type : Original Article

Authors

1 Pharmaceutical technology unit, National Institute of Laser Enhanced Sciences (NILES), Cairo University

2 Dept. Medical Applications of Laser, Pharmaceutical Nanotechnology unit, National Institute of Laser Enhanced Science , Cairo University

Abstract

Purpose: In this study, the efficacy of a gel formulation containing Hp-HCl (a photosensitizer) dissolved in glycerin was evaluated for photodynamic treatment (PDT) of tumors.
Methods: The release kinetics of water-dissolved Hp-HCl and glycerin-dissolved Hp-HCl from gel formulae were studied in vitro, where the latter showed faster drug release. The growth rates of tumor size in animals, from different treatment groups, were measured.
Results: The group treated with the glycerin-dissolved Hp-HCl showed more significant decrease in tumor rate (smaller size) in comparison to the water-dissolved formula, after 35 post-implantation days. Histopathological examination of tumor sections revealed that glycerin-dissolved Hp-HCl effectively distributed the drug throughout the tumor, resulting in almost complete disappearance of tumor cells. Overall, the dissolution of Hp-HCl in glycerin not only increased drug release from the gel formulation but also improved its distribution and therapeutic efficiency. These findings were supported by histopathological results
Conclusion: Suggesting that the topical use of Hp-HCl dissolved in glycerin holds promise for improved PDT.
 
 

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